What is included in a Disinfection check?

Purpose Disinfection check:

Check the effectiveness of the disinfection.

Definition Disinfection check:

Disinfection: killing micro-organisms with the help of a disinfection solution. 

Frequency: See specification /overview.

Implementation:

A good disinfection is very important to prevent the accumulation of micro-organisms. It also prevents the possibility of cross contamination. Through proper disinfection, the micro-organisms are killed. The following can be done yourself or (partially) outsourced. 

• Make an petri dish of the object to be assessed. 
• Make sure the agar is not touched. This causes contamination and gives a wrong result. 
• Clearly label the petri dish. 
• Incubate the petri dish according to specifications. 
• Assess the petri dish. 
• Record the results. 

Assess petri dish:

Normally the results are sorted by a classification:

Class	Amount CFU	Verdict
0	0-2	Very good
1	3-9	Good
2	10-29	Moderate
3	30-89	Poor
4	90 and higher	Very poor

Because we only view 0 and 1 as good, we have chosen to use the following classification: 

Class	Amount CFU	Verdict
0	10 and more	Poor
1	0-9	Good

Actions by abnormalities:

When a 0 is scored, a resampling should be performed within 1 week to assess whether it is a structural abnormality or an incident. If the resampling is still poor: 

• The cause must be determined, for example: 

o ignorance; 
o Incorrect instructions. 

• The cleaning execution should be checked. 
• The personnel should be instructed regarding cleaning and disinfection. 
• After instruction, a new sampling should take place. 
• If more than 50% of the items (total) scores a 0 for the first sample and resampling, the supplier of the disinfectants must be contacted. This is to prevent microbiological resistance. 
• Record all measures taken.